Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp

Abstract Banana production generates significant amounts of agricultural wastes, being fiber extraction one the most relevant alternatives for their valorization. This process produces banana’s pseudostem pulp (BPP) as a byproduct, which shows an interesting composition biorefinery’s biochemical platform, with high polysaccharides (68%) and low lignin contents. work deals enzymatic hydrolysis (EH) raw hydrothermally pre-treated BPP, focusing on oligosaccharides (OS). Raw BPP cellulase at different dosages rendered only 3.2% OS yields (OSY). Pectinase addition has not affected EH performance. On other hand, 150 °C 170 (P150 P170) allowed to increase OSY up 28% (P150, 1 FPU cellulase/g dry biomass, 12 h), 72% solubilized sugars in form cello-oligosaccharides. last condition was subjected multi-stage strategy without improvements OSY. An endo-glucanase also tested, but obtained were lower than results. Finally, demonstrated stimulate growth two Lactobacilli strains. The results show that under mild operational conditions is good candidate cello-oligosaccharides by using FPU/g DB simple strategy. Graphical abstract